Antibacterial, Antioxidant and Cytotoxic Activities of Heliotropiumindicum

Heliotropium indicum has many traditional medicinal uses. The present study is based on this plant to evaluate the antibacterial,
antioxidant and cytotoxic activities. H. indicum was subjected to bacterial investigation by disc diffusion method against Gram positive
and Gram negative bacteria using kanamycin as standard. The methanolic extract of the plant showed good antibacterial activity against Pseudomonas aeruginosa with 15 mm zone of inhibition. The extract showed mild to moderate antibacterial activity against
Staphylococcus aureus, Bacillus subtilis and Bacillus megaterium with 10 mm, 11mm and 12 mm zone of inhibition. Antioxidant activity of the plant was evaluated spectrophotometrically using DPPH and ascorbic acid as standard. The extract showed significant antioxidant activity with IC50 of 58.30 μg/ml whereas IC50 of ascorbic acid was 45.74 μg/ml. The cytotoxic activity of H. indicum was determined by brine shrimp lethality bioassay using vincristine sulfate as standard. The meathanolic extract showed significant cytotoxic activity with LC50 of 6.607 μg/ml whereas, LC50 of Vincristine sulphate was 6.026 μg/ml.