Development and validation of novel HPLC Method for Simultaneous determination of seven compounds(Alpha Pinene, Beta Pinene, Camphene, Pinene linalool, safrole, Anisaldehyde, Acetoanisole) in Anise essential oil.

New HPLC method was developed and validated according to ICH guidelines to analyze seven major chemical
constituents Alpha Pinene, Beta Pinene, Camphene, pinene linalool, safrole, Anisaldehyde, Acetoanisole
simultaneouslyin Anise essential oil. This method is one of the alternative method for LC-ME method.