Acorus calamus L. is an important and threatened medicinal plant with tremendous potential as an important natural resource and has
high demand in the world market due to wide range of applications. The development of conservation and commercialization technique is a priority at present. F-factor analysis showed that different treatments of growth hormones play a major role in shoot regeneration. Sprouting shoots from rhizome explants were collected from field-grown plants and 93.3% explants were responded in Murashige and Skoog’s medium supplemented with 0.5 mg l-1 IAA and 1.0 mg l-1 BA but after two weeks explants expressed bacterial contamination. Among all antibiotics only, Augmentin® antibiotic was not only controlled bacterial contamination but also supported the regeneration of shoots. Shoot organo genesis could also be induced when basal leaf explants of in vitro-grown shoots were incubated in a medium containing MS + 2.5 mg l-1 BA + 1.0 mg l-1 NAA and 91.3 % explants were responded. LSD analysis shows that this concentration is highly significant among all treatments. Well differentiated shoots were observed within four weeks of culture incubation. The regenerated shoots were further elongated even in one tenth lower concentration of BA (0.25 mg l-1) along with 0.25 mg l-1 GA and 0.1 mg l-1 IAA. Isolated grown-up shoots were rooted in the p resence of 0.5 mg l-1 IAA, hardened and transferred in the pots.
Abstract