A simple and inexpensive method was developed using solid-phase extraction, together with high performance liquid chromatographic method with UV detection for determination of tolfenpyrad residues. The evaluated parameters include the extracts by fluorosil packed column using ethyl acetate: cyclohexane (9:1) and acetonitrile: HPLC grade water (9:1) mixture of solvents. The method was validated using mango fruit samples spiked with tolfenpyrad at different fortification levels (0.03 and 0.3 µg/g). Average recoveries (using each concentration six replicates) ranged 84-93%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.01-10.0 µg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.01µg/g and 0.03µg/g respectively. Finally the mango fruit residue samples were re analyzed by HPLC.
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