A Novel Method for Determination of Cyclanilide and its Metabolite Residues in Cotton Seed Oil

A simple, sensitive and inexpensive method was developed using solid-phase extraction, together with high performance liquid
chromatographic method with uv detection for determination of cyclanilide and its metabolite (2, 4 dichloroaniline) residues. The
evaluated parameters include the extracts by gel permeation chromatography (clean-up module GPC) on bio beads S-X3 polystyrene gel using a mixture of ethyl acetate/ cyclohexane (1:1, v/v) as eluent (5.0 mL/min) and an automated gel permeation chromatography. The method was validated using cotton oil samples spiked with cyclanilide and its metabolite (2,4 dichloroaniline) at different concentration levels (0.05 and 0.5 μg/mL). Average recoveries (using each concentration six replicates) ranged 89-95%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.05-5.0 μg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.02 μg/mL and 0.05 μg/mL respectively.