Issue
The Experiment 2012
Article Category
Research Article
Article Subject
Pharmacy
Article Authors
ANUSHA DEVI.V
VIJAYA KUMARI.M
AFROZ PATAN
Abstract

A simple RP-HPLC method for the determination of Glatiramer acetate in pharmaceutical dosage forms. Numerous HPLC conditions
were tested for determination of Glatiramer acetate. The best result was achieved by using Develosil 300, C-4 (250×4.6mm) 5μm
column and a mobile phase consisting of Acetonitrile: Trifluoroacetic acid at flow rate of 1.0 ml/min with ultraviolet detection at
220nm. The retention time of the drug was 9.039 min. The method produced liner responses in the concentration range of 8 to 12 ppm of Glatiramer acetate. The method was found to be applicable for determination of the drug in injection.

Keywords
Glatiramer acetateEstimation
RP-HPLC
validation
Acetonitrile
Trifluoro acetic acid.
Article PDF
PDF (For Download)