Article Subject

Clopidogrel and atorvastatin are available in capsule formulation to treat heart related diseases and abnormalities. Simple
HPLC method was developed and validated. Chromatographic conditions are Mobile phase: 1ml of OPA solution in a
1000ml of of water and mix the above OPA solution with acetonitrile in the ratio of 60:40 v/v; Flow rate: 1 ml/min; Column:
Discovery C18 150 x 4.6 mm, 5m; Detector wave length: 240nm; Column temperature: 30°C; Injection volume: 10mL; Run
time: 6min; Diluent: Water: Acetonitrile (50:50). Method validation results were confirmed the method extendedness with
precision % RSD, linearity correlation coefficient above 0.999, accuracy recovery between 98% to 102%, specificity
interference, peak purity and % degradation, ruggedness and robustness results. Eventually the finalized method can be used for the determination of Clopidogrel and atorvastatin in capsule formulation.

HPLC Method
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