Article Subject

A simple, selective, linear and accurate isocratic RP-HPLC method has been developed for the estimation of
Regorafenib in and tablet dosage forms. The chromatographic separation was achieved on C18, 250×4.60mm
5micron particle size column. The mobile phase consists of a mixture of acetonitrile and Methanol (pH 5.2) in
the ratio of 45:55% v/v with UV-detection at 261 nm. The flow rate was kept at 1mL/min. The method shows a
linear response for concentrations in the range of 5-25μg/mL. The developed method was statistically validated
for Accuracy, linearity, precision, robustness, LOD and LOQ.

Regorafenib C18 column
method development and validation
261 nm
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