Single RP-HPLC Method for the Quantification of Candesartan and Hydrochlorothiazide in Formulations

Objectives: To develop a single RP-HPLC method for determination of candesartan and hydrochlorothiazide in formulation.
Methods: Chromatographic separation was achieved on a C18, 250 x4.6mm, 5μ column. Mobile phase composed of methanol,
acetonitrile and 0.1% Ortho phospharic acid in the ratio of 35:50:15v/v with 1.0ml per min flow rate and detection was at 272 nm.
Injection volume is 10μL and run time was selected for 12min. Results: High resolution was achieved and retention time of
hydrochlorothiazide and Candesartan are about 4.58min and 6.94min, respectively. The absorbance of both active peaks were a
linear function of concentration in the range 30-90 for both hydrochlorothiazide and Candesartan. The correlation co-efficient value
also within the limit (0.999). Conclusions: Proposed single HPLC method was validated with specificity, linearity, accuracy,
reproducibility and ruggedness. The method has applicable for regular analysis.