Stable and novel HPLC Method for the determination of Clopidogrel and Atorvastatin In Capsule formulation

Clopidogrel and atorvastatin are available in capsule formulation to treat heart related diseases and abnormalities. Simple
HPLC method was developed and validated. Chromatographic conditions are Mobile phase: 1ml of OPA solution in a
1000ml of of water and mix the above OPA solution with acetonitrile in the ratio of 60:40 v/v; Flow rate: 1 ml/min; Column:
Discovery C18 150 x 4.6 mm, 5m; Detector wave length: 240nm; Column temperature: 30°C; Injection volume: 10mL; Run
time: 6min; Diluent: Water: Acetonitrile (50:50). Method validation results were confirmed the method extendedness with
precision % RSD, linearity correlation coefficient above 0.999, accuracy recovery between 98% to 102%, specificity
interference, peak purity and % degradation, ruggedness and robustness results. Eventually the finalized method can be used for the determination of Clopidogrel and atorvastatin in capsule formulation.