Synthesis and Characterization of Silver Nanoparticles using aqueous extract of Andrographis paniculata and their Antimicrobial Activities

Silver nanoparticles (Ag-NPs) have been successfully prepared with simple and green synthesis method by reducing Ag+ ions in aqueous extract as a reducing agent. In this study, aqueous extract of A. paniculata was used as a reducing and stabilizing agent. The structural and antimicrobial properties of the synthesized nanoparticles were studied. X-ray diffraction patter (XRD) pattern showed the crystalline nature of particles. The average diameter of the nanoparticles was calculated by TEM and XRD was around 83nm. Energy dispersive X-ray spectroscopy (EDS) spectrum and XRD pattern suggested that prepared nanoparticles were highly pure. The nanoparticles showed excellent antimicrobial activity against various bacterial. Moreover, P. aeruginosa exhibited the highest sensitivity to nanoparticles while B. Subtilis was the least sensitive.