Antibiotic resistance is nowadays the major concern of many clinicians. This phenomenon is at the origin of a therapeutic impasse with high rates of morbidity and mortality. This calls for alternative care alternatives. The present study was initiated with the aim of evaluating the antibacterial activity of the aqueous and ethanolic extract 70% of Garcinia kola seeds on the in vitro growth of β-lactamase producing strains. The methodology consisted of extracting the drugs with a 70% hydroalcoholic solvent and distilled water. The diffusion methods in agar medium and in liquid medium were used for the sensitivity test and the determination of the MIC and the CMB. Inhibition zone diameters varied from 10 to 15.66 mm, however, the MICs of the extracts ranged from 3.12 mg / mL to 25 mg / mL and the CMBs ranged from 6.25 mg / mL to 100 mg / mL. The lowest value of MIC and CMB was observed with E. coli ATCC 25922 while the largest value of these same parameters was obtained on S. flexneri and S. typhi. The ethanol extract 70% and the oxacillin (reference molecule) exerted a bactericidal action on all the strains whereas, the aqueous extract recorded a bactericidal activity on the strains of E. coli and a bacteriostatic activity on S. flexneri and S. typhi. In addition, the antibacterial activities of Garcinia kola seeds highlighted in this study justify their use in the treatment of various infectious diseases in traditional environment.