Abstract

A Simple fast and precise reversed phase high performance liquid chromatographic method is developed for the Deflazacort drug. Chromatographic separation was performed a C18 column (250×4.6 mm) as a stationary phase with the mobile phase of Acetonitrile, methanol and water (90:5:5 v/v). Flow rate is 1.0 ml/min the detection of wave length is 230 nm. This method was validated for linearity, accuracy, precision, LOD & LOQ as per the guidelines of ICH. The percentage of recovering was 98.8.The developed method was applied successfully for determination of Deflazacort is pharmaceutical dosage form.

Issue

The Experiment 2012

Article Subject:

Pharmacy

KeyWords:

High performance liquid Chromatographic method, Deflazacort, ICH, Stationary Phase

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R.Ramesh Raju et al, The Experiment, JULY.2012 Vol.1 (1), 29-34