Abstract
A Simple fast and precise reversed phase high performance liquid chromatographic method is developed for the Deflazacort drug. Chromatographic separation was performed a C18 column (250×4.6 mm) as a stationary phase with the mobile phase of Acetonitrile, methanol and water (90:5:5 v/v). Flow rate is 1.0 ml/min the detection of wave length is 230 nm. This method was validated for linearity, accuracy, precision, LOD & LOQ as per the guidelines of ICH. The percentage of recovering was 98.8.The developed method was applied successfully for determination of Deflazacort is pharmaceutical dosage form.