A Novel RP-HPLC Method for the Determination of Venlifaxine in Pharmaceutical Drug Products

Abstract

Sensitive, simple and accurate high performance liquid chromatographic (HPLC) methods for the determination of Venlafaxine in bulk drug and pharmaceutical dosage form. The quantification was carried out using C 18 (chromosil, 250mm, 4.6mm) column and mobile phase comprised of Water, Tri ethyleamine, 1% Ortho Phosphoric acid in proportion of 50:30:20 (v/v/v) at pH 4.2. The flow rate was 1.5 ml/min and the effluwas monitored at 245 nm. The retention time of Venlafaxine is 4.0min. The method was validated in terms of linearity, precision, accuracy, and specificity, limit of detection and limit of quantitation. Linearity of Venlafaxine is 3- 21ppm. The percentage recovery is 99.85% from the tablet formulation. The proposed method is suitable for simultaneous determination of pioglitazone and glimepiride in pharmaceutical dosage form and bulk drug.