Abstract
A simple RP-HPLC method for the determination of Glatiramer acetate in pharmaceutical dosage forms. Numerous HPLC conditions were tested for determination of Glatiramer acetate. The best result was achieved by using Develosil 300, C-4 (250×4.6mm) 5μm column and a mobile phase consisting of Acetonitrile: Trifluoroacetic acid at flow rate of 1.0 ml/min with ultraviolet detection at 220nm. The retention time of the drug was 9.039 min. The method produced liner responses in the concentration range of 8 to 12 ppm of Glatiramer acetate. The method was found to be applicable for determination of the drug in injection.
Issue
The Experiment 2012
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KeyWords:
Glatiramer acetateEstimation, RP-HPLC, validation, Acetonitrile, Trifluoro acetic acid